Page 158 - 《环境工程技术学报》2023年第1期

Vol.13，No.1 环 境 工 程 技 术 学 报 第 13 卷，第 1 期
Jan.，2023 Journal of Environmental Engineering Technology 2023 年 1 月

曹洋，孙鹤铭，刘利，等.冬季衡水湖沉积物微生物群落结构特征及影响因 素 [J].环境工程技术学报，2023，13（1）：154-163.
CAO Y,SUN H M,LIU L,et al.Microbial community structure characteristics and influencing factors in sediments of Hengshui Lake in winter[J].Journal
of Environmental Engineering Technology，2023，13（1）：154-163.

冬季衡水湖沉积物微生物群落结构特征及影响因素

曹洋 ，孙鹤铭 ，刘利 ，敖蒙蒙 ，王俭 ，魏健 2*
1
1,2
1
1
1*
1.辽宁大学环境学院
2.中国环境科学研究院水生态环境研究所
摘要 湖泊沉积物中的微生物对有机物和营养盐的转化起着重要作用，其群落结构也会受环境因子的影响。为探究冬季衡水湖
沉积物中微生物群落结构差异及影响因素，基 于 16S rRN 基因高通量测序，分析衡水湖不同湖区表层沉积物中微生物群落结
A
构组成、多样性及与环境因子之间的响应关系。结果表明：不同湖区沉积物中微生物群落多样性表现为湖北区>湖心区>
湖南区，湖北区与湖心区沉积物的微生物群落结构存在极显著差异（P<0.01）。在门水平上，湖心区沉积物中绿弯菌门
（Chloroflexi）的相对丰度显著高于其他湖区，这与湖心区有机污染严重有关；在属水平上，湖北区沉积物 中 P9X2b3D0 相对丰
2
度显著高于其他湖区，说明该菌属更适宜在水生植物丰富的环境下生长繁衍。有机碳（TOC）浓度是衡水湖水体沉积物微生物群
落结构的关键影响因素，TO 浓度与微生物菌属相对丰度的高相关性与沉积物有机污染严重有关。
C
关键词 衡水湖；沉积物；微生物；群落结构；生境异质性
中图分类号：X524,X172 文章编号：1674-991X（2023）01-0154-10 doi：10.12153/j.issn.1674-991X.20210652

Microbial community structure characteristics and influencing factors in
sediments of Hengshui Lake in winter

1
1
1
1,2
1*
CAO Yang , SUN Heming , LIU Li , AO Mengmeng , WANG Jian , WEI Jian 2*
1.College of Environmental Sciences, Liaoning University
2.Institute of Water Ecology and Environment, Chinese Research Academy of Environmental Sciences
Abstract The microorganism of lake sediments plays an important role in the transformation of organic matter and
nutrients, and the microbial community structure is also affected by various environmental factors. In order to
explore the differences of the microbial community structure and the influencing factors in the sediments of
Hengshui Lake in winter, based on 16S rRNA gene high-throughput sequencing, the microbial community structure
composition, diversity and response relationship with environmental factors in various lake areas were analyzed.
The results showed that the microbial community diversity in the sediments of various lake areas was in the order of
the north area of lake>the center area of lake>the south area of lake. There were extremely significant differences
(P<0.01) in the microbial community structure of sediments in the north area of lake and the center area of lake. At
the phylum level, the relative abundance of Chloroflexi in the sediment samples in the center area of lake was
significantly higher than that in other lake areas, which was related to the serious organic pollution in the center area
of lake. At the genus level, the relative abundance of P9X2b3D02 in sediment samples of the north area was
significantly higher than that in other areas, indicating that this genus was more suitable for growth and reproduction
in an environment with rich aquatic plants. The total organic carbon (TOC) content of the sediments was the key
influencing factor of microbial community structure in lake sediments. The high correlation between TOC
concentration and the relative abundance of microbial species was related to the heavy organic pollution of the
sediments.
Key words Hengshui Lake; sediments; microorganism; community structure; habitat heterogeneity

收稿日期：2021-11-09
基金项目：国家水体污染控制与治理科技重大专 项 (2017ZX07401-004)
作者简介：曹洋（1997—），女，硕士研究生，主要研究方向为水污染控制与治理，CY19970920@163.com
* 责任作者：1.刘 利 (1981—)，男， 副教授，博士，主要从事水环境治理与生态修复技术研究，liul215@163.com
2.魏 健 (1983—)，男，副研究员，博士，主要从事水污染控制技术研究，weijian0911@163.com