Page 284 - 《环境工程技术学报》2022年第5期

Vol.12，No.5 环 境 工 程 技 术 学 报 第 12 卷，第 5 期
Sep.，2022 Journal of Environmental Engineering Technology 2022 年 9 月

熊江磊，罗嘉豪，严群.改性蓝藻生物炭促进生物电化学系统阴极氢自养反硝化过程研 究 [J].环境工程技术学报，2022，12（5）：1640-1646.
XIONG J L,LUO J H,YAN Q.Research on modified cyanobacterial biochar promoting cathodic hydrogen autotrophic denitrification in bioelectrochemical
system[J].Journal of Environmental Engineering Technology，2022，12（5）：1640-1646.

改性蓝藻生物炭促进生物电化学系统阴极氢

自养反硝化过程研究

熊江磊 ，罗嘉豪 ，严群 2
1
1
1.中国电子系统工程第二建设有限公司
2.江南大学环境与土木工程学院
摘要 以太湖蓝藻为原料制备不同种类的活性生物炭，并将其投入到生物电化学系统（BES）的阴极促进氢自养反硝化。通过扫
描电镜、能谱仪和傅里叶红外光谱对未经改性（ABC-800）、硝酸改性（ABC-800N） 和 KO 改性（ABC-800K） 组蓝藻生物炭进
3
H
行观察，并与不加入蓝藻生物炭的对照组进行比较，以考察生物炭促 进 BE 生物阴极的反硝化过程中的电子传递机制。结果表
S
明：ABC-800 表面 的 N、 元素含量最高，同时与电子传递能力及生物相容性相关的共轭醌、酮结构的丰度也最高；将蓝藻生
N
O
物炭投加 至 BE 的非生物阴极中可提高阴极的脱氮效率，ABC-800 投加量 为 0.5 时，7 内脱氮效率达到最高， 为 96.0%，而
N
d
S
g
对照组仅 为 29.6%；高通量测序表明，ABC-800 组的优势菌属 为 Thauera、JGI_0001001_H03、Thiobacillus、Denitratisom 等。
a
N
关键词 生物电化学系 统 (BES)；氢自养反硝化；蓝藻生物炭；高通量测序
中图分类号：X52,X703 文章编号：1674-991X（2022）05-1640-07 doi：10.12153/j.issn.1674-991X.20210362
Research on modified cyanobacterial biochar promoting cathodic hydrogen
autotrophic denitrification in bioelectrochemical system
1
1
XIONG Jianglei , LUO Jiahao , YAN Qun 2
1.China Electronics System Engineering NO.2 Construction Co., Ltd.
2.School of Environmental and Civil Engineering, Jiangnan University
Abstract Different kinds of active biochar were prepared from cyanobacteria in Taihu Lake and put into the
cathode of bioelectrochemical system (BES) to promote hydrogen autotrophic denitrification. Three groups of
cyanobacterial biochar of Unmodified (ABC-800), modified by nitric acid (ABC-800N) and modified by KOH
(ABC-800K) were observed by scanning electron microscopy (SEM), energy dispersive spectrometer (EDS) and
Fourier transform infrared spectroscopy (FTIR), and were compared with the control group without the addition of
cyanobacteria biochar, to investigate the electron transfer mechanism in denitrification process of biochar promotes
BES biocathode. The results showed that the contents of nitrogen and oxygen on ABC-800N surface were the
highest, and the abundance of conjugated quinone and ketone structures related to electron transport capacity and
biocompatibility was also the highest. Adding cyanobacteria biochar to the abiotic cathode of BES could improve
the nitrogen removal efficiency of the cathode. After adding 0.5 g ABC-800N into the BES abiotic cathode, the
nitrogen removal efficiency reached the highest within 7 days, which was 96.0%, while the control group was only
29.6%. High-throughput sequencing showed that the dominant bacteria in ABC-800N group were Thauera,
JGI_0001001_H03, Thiobacillus, and Denitratisoma, etc.
Key words bioelectrochemical system(BES); hydrogenotrophic denitrification; cyanobacterial biochar; high-
throughput sequencing

含硝酸盐废水的过量排放和不当处置可能导致 生物处理法等常规处理手段相比，利用生物电化学
出生缺陷、致畸性和发生突变等不良影响，故这类废 系统（BES）处理废水对能耗及污泥处理的需求更
[2]
水的有效处理已受到广泛关注 。与物理化学法或 低 。此外，与以硫、铁及其化合物为底物的自养反
[1]

收稿日期：2021-07-29
基金项目：江苏省“双创博士”企业创新类人才项目（〔2019〕3027 号）；江苏省“双创人才”项目（〔2020〕1034 号）
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9
作者简介：熊江磊（1984—），男，高级工程师，博士，研究方向为工业废水的处理及资源化，xiongjianglei@hotmail.com