Page 209 - 《环境工程技术学报》2023年第1期

Vol.13，No.1 环 境 工 程 技 术 学 报 第 13 卷，第 1 期
Jan.，2023 Journal of Environmental Engineering Technology 2023 年 1 月

李朝明，许丹，黄铭意，等.不同阳极设置对人工湿地-微生物燃料电池脱氮及产能的影 响 [J].环境工程技术学报，2023，13（1）：205-213.
LI C M,XU D,HUANG M Y,et al.Effects of different anode settings on the performance of nitrogen removal and electrogenesis capacity in constructed
wetland-microbial fuel cells[J].Journal of Environmental Engineering Technology，2023，13（1）：205-213.

不同阳极设置对人工湿地-微生物燃料电池

脱氮及产能的影响

李朝明，许丹，黄铭意，唐顺静，韩胡威
东华理工大学水资源与环境工程学院

摘要 人工湿地-微生物燃料电池（constructed wetland-microbial fuel cell, CW-MFC）耦合系统是人工湿地和生物电化学技术的有
机结合，其中阳极是限制耦合系统输出功率和污染物净化性能的关键因素。构建了未加入颗粒活性炭（CW-MFC1）和加入颗粒
活性炭（CW-MFC2） 套耦合系统以探讨阳极加入颗粒活性炭对耦合系统产电和脱氮性能的影响，并利用高通量测序技术对比
2
2
分 析 2 套系统阳极和阴极微生物群落组成。结果表明：CW-MFC 耦合系统的输出电压和最大功率密度（430 mV，8.39 mW/m ）
2
高 于 CW-MFC1（379 mV，7.77 mW/m ）。试验运行前期（0 ～29 d），CW-MFC 耦合系统的氨氮去除率 为 65.72%±3.06%，显著高
2
2
于 CW-MFC1（56.06%±3.71%），而二者的总氮去除率相差不大；随着时间的推移（30 ～105 d），CW-MFC 耦合系统的氨氮和总
1
氮去除率逐渐高于 CW-MFC2，尤其是总氮去除更为显著（CW-MFC 为 42.69%±4.19%，CW-MFC 为 32.50%±11.51%）。
2
1
高通量测序结果表明，CW-MFC 阳极富集的不动杆菌属以及阴极大量的反硝化菌（巨大芽殖杆菌属、地杆菌属、黄杆菌属、不
1
动杆菌属和脱氯单胞菌属等）的富集可能是其脱氮性能优 于 CW-MFC 的主要原因。综上，阳极加入颗粒活性炭可提 升 CW-
2
MF 耦合系统的产电性能，但不利于生物脱氮过程。
C
关键词 人工湿地；微生物燃料电池；脱氮；颗粒活性炭；阳极
中图分类号：X703 文章编号：1674-991X（2023）01-0205-09 doi：10.12153/j.issn.1674-991X.20220048
Effects of different anode settings on the performance of nitrogen removal and
electrogenesis capacity in constructed wetland-microbial fuel cells
LI Chaoming, XU Dan, HUANG Mingyi, TANG Shunjing, HAN Huwei
School of Water Resources and Environmental Engineering, East China University of Technology

Abstract Constructed wetland-microbial fuel cell (CW-MFC) coupling system is a combination of constructed
wetland and bioelectrochemical technology, in which the anode is the key factor to limit the output power and
pollutant purification performance of the coupling system. Two coupling systems, CW-MFC1 (without granular
activated carbon) and CW-MFC2 (with granular activated carbon), were constructed to investigate the effects of the
addition of granular activated carbon at the anode on the power generation and nitrogen removal performance of the
coupling system. The compositions of microbial communities at the anode and cathode of the two systems were
analyzed by high-throughput sequencing technology. The results showed that the output voltage and maximum
2
power density (430 mV, 8.39 mW/m ) of the CW-MFC2 reactor were higher than those of CW-MFC1 (379 mV,
2
7.77 mW/m ). The ammonia nitrogen removal rate of the CW-MFC2 reactor was 65.72%±3.06% in the early stage
of the experimental operation (days 0 to 29), which was significantly higher than that of CW-MFC1
(56.06%±3.71%), and the total nitrogen removal rates of the two were relatively close. On days 30 to 105, the
ammonia nitrogen and total nitrogen removal rate of the CW-MFC1 reactor were gradually higher than that of
CW-MFC2, especially the total nitrogen removal was more significant (CW-MFC1 42.69%±4.19%, CW-MFC2
32.50%±11.51%). Besides, the high-throughput sequencing results showed that the high abundance of Acinetobacter
at the anode and plentiful denitrifying bacteria (Gemmobacter, Geobacter, Flavobacterium, Acinetobacter , and
Dechloromonas, etc.) in CW-MFC1 might be the main reasons for the better denitrification performance of

收稿日期：2022-01-17
基金项目：江西省自然科学基金项 目 (20192BAB213021)
作者简介：李朝明（1982—），男，讲师，硕士，主要从事水及污水处理技术理论研究，cmli@ecut.edu.cn